Physiologie I: Kardiovaskuläre Physiologie -
Prof. Dr. Ralf Brandes

Expertise

Mechanismen der vaskulären Redoxregulation
Lipidsignaling im Gefäßsystem
Vaskuläre Autacoidproduction und Autacoideffekte
Pathomechanismen der vaskulären Krankheitsentwicklung
Integrative Physiologie

Methoden und Geräte

Integrative Physiologie in Mäusen (Modelle der Atherosclerose, Hypertonie, Schlaganfall, progressiven Niereninsuffizienz, Telemetrie, Hpyoxie)
Bestimmung der vaskulären und zellulären Produktion von reaktiven Sauerstoffspezies (ROS) und deren biologischer Effekte
Zellkulturmodell zur Monolayerpermeabilität, Zellmigration & Angiogenese
Kultur primärer Zellen aus Blutgefäßen und Gehirn.

Publikationen

Heumüller S, Wind S, Barbosa-Sicard E, Schmidt HH, Busse R, Schröder K, Brandes RP: Apocynin is not an inhibitor of vascular NADPH oxidases but an antioxidant. Hypertension 2008; 51:211-217.
Schröder K, Helmcke I, Palfi K, Krause K.-H., Busse R, Brandes RP: Nox1 mediates basic fibroblast growth factor-induced migration of vascular smooth muscle cells Arterioscler Thromb Vasc Biol 2007; 27:1736-1743.
Kahles T, Luedike P, Endres M, Galla H-J, Steinmetz H, Busse R, Neumann-Haefelin T, Brandes RP: NADPH oxidase plays a central role for blood-brain barrier damage in experimental stroke, Stroke 2007; 38:3000-3007.
Büssemaker E, Pistrosch F, Forster S, Herbrig K, Gross P, Passauer J, Brandes RP: Rho-kinase contributes to basal vascular tone in man – role of endothelium-derived nitric oxide. Am J Physiol Heart Circ Physiol 2007; 293:H541-547.
Jung O, Marklund SL, Xia N, Busse R, Brandes RP: inactivation of extracellular superoxide dismutase contributes to the development of high-volume hypertension. Arterioscler Thromb Vasc Biol 2007; 27:470-477.
Schröder K, Vecchione C, Jung O, Schreiber JG, Schiri-Sverdlov R, van Gorp P, Busse R, Brandes RP: The xanthine oxidase inhibitor tungsten prevents the development of atherosclerosis in ApoE knockout mice fed a Western-type diet. Free Radical Bio Med 2006; 141:1353-1360.
Ambasta RK, Schreiber JG, Janiszewski M, Busse R, Brandes RP: Noxa1 is a central component of the smooth muscle NADPH oxidase in mice, Free Radical Biol Med 2006; 41:193-201.
Jung O*, Brandes RP*, Kim IH, Schweda F, Schmidt R, Hammock BD, Busse R, Fleming I. The soluble epoxide hydrolase is a main effector of angiotensin II-induced hypertension, Hypertension 2005; 45:1-7. *both authors contributed equally to this work
Ambasta RK, Kumar P, Griendling KK, Schmidt HHHW, Busse R, Brandes RP, Direct interaction of the novel Nox proteins with p22phox is required for the formation of a functionally active NADPH oxidase, J Biol Chem 2004; 479:45935-45941.
Jung O, Schreiber JG, Geiger H, Pedrazzini T, Busse R, Brandes RP. The gp91phox-containing NADPH oxidase mediates endothelial dysfunction in renovascular hypertension. Circulation 2004; 109:1795-1801.
Jung O, Marklund SL, Geiger H, Pedrazzini T, Busse R, Brandes RP. Extracellular superoxide dismutase is a major determinant of nitric oxide bioavailability. In vivo and ex vivo evidence from ecSOD-deficient mice. Circ Res. 2003; 93:622-629.
Brandes RP, Beer S, Ha T, Busse R. Withdrawal of cerivastatin induces monocyte chemoattractant protein 1 and tissue factor expression in cultured vascular smooth muscle cells. Arterioscler Thromb Vasc Biol. 2003; 23:1794-1800.
Büssemaker E, Popp R, Fisslthaler B, Larson CM, Fleming I, Busse R, Brandes RP. Aged spontaneously hypertensive rats exhibit a selective loss of EDHF-mediated relaxation in the renal artery. Hypertension 2003; 42:562-568.
Büssemaker E, Popp R, Fisslthaler B, Larson CM, Fleming I, Busse R, Brandes RP. Hyperthyroidism enhances endothelium-dependent relaxation in the rat renal artery. Cardiovasc. Res. 2003; 59:181-188.
Vecchione C, Brandes RP. Withdrawal of 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors elicits oxidative stress and induces endothelial dysfunction in mice. Circ Res 2002; 91:173-179.