Klinik für Gynäkologie und Geburtshilfe -
Prof. Dr. med. Manfred Kaufmann

Expertise

Genexpressionsanalyse maligner gynäkologischer Tumoren
Differenzierung biologischer Marker beim Mammakarzinom
Endokrin vermittelte Regulationsmechanismen des Mammakarzinoms
Methylierungsanalysen
Hemmung verschiedener Gene (PLK1 [polo-like kinase 1], Cyclin B1, Her2/neu u.v.m.) mittels siRNA

Methoden und Geräte

Breites Spektrum molekularbiologischer Techniken für die Analyse von Nukleinsäuren und Proteinen (Northern-, Southern- und Western-Blot, PCR, Klonierung)
2D-Gelelektrophorese
DNA-chip-Analyse
Hochselektive PCR-Analyse zum Nachweis von Methylierungsmustern
Proliferationsassays
Transfektion (siRNA, Antisense-Oligos, Plasmide)
Transformation
Kinaseassays
Immunfluoreszenz/Immunhistologie
(Co)-Immunpräzipitation
FACScan-Analyse

Tiereperimentelle Methoden:

humane Xenograft-Experimente an Nacktmäusen und anschließende molekularbiologische Analyse der Tumoren/Organe
Herstellung transgener Tiere

Publikationen

Spänkuch,B, Matthess,Y., Knecht,R., Zimmer,B., Kaufmann,M., Strebhardt,K. 2004. Cancer inhibition in nude mice after systemic application of U6 promoter-driven siRNAs targeted against PLK1. J. Natl. Cancer Inst. 96, 862-872.
Yuan,J., Yan,R., Kramer,A., Eckerdt,F., Roller,M., Kaufmann,M., Strebhardt,K. 2004. Cyclin B1 depletion inhibits proliferation and induces apoptosis in human tumor cells. Oncogene 23, 5843-52.
Spänkuch-Schmitt,B., Wolf,G., Solbach,C., Loibl,S., Knecht,R., Stegmüller,M., von Minckwitz,G., Kaufmann,M., Strebhardt,K. 2002. Downregulation of human polo-like kinase activity by antisense oligonucleotides induces growth inhibition in cancer cells. Oncogene 21, 3162-3171.
Spänkuch-Schmitt,B., Bereiter-Hahn,J., Kaufmann,M., Strebhardt,K. 2002. Effect of RNA Silencing of Polo-Like Kinase-1 (PLK1) on Apoptosis and Spindle Formation in Human Cancer Cells. J Natl. Cancer Inst. 94, 1863-1877.
Yuan,J., Kramer,A., Eckerdt,F., Kaufmann,M., Strebhardt,K. 2002. Efficient internalization of the polo-box of polo-like kinase 1 fused to an Antennapedia peptide results in inhibition of cancer cell proliferation. Cancer Res. 62, 4186-4190.
Strebhardt,K., Spänkuch-Schmitt,B. 2002. Hemmung von Zellzyklus-Regulatoren in der gynäkologischen Therapie. In: Gynäkologie und Geburtshilfe Frankfurt 2002. Hrsg. Kaufmann,M., Ahr,A. sympomed Verlag, München.
Yuan,J., Eckerdt,F., Bereiter-Hahn,J., Kurunci-Csacsko,E., Kaufmann,M., Strebhardt,K. 2002. Cooperative phosphorylation including the activity of polo-like kinase 1 regulates the subcellular localization of cyclin B1. Oncogene 21, 8282-92.
Ahr A, Karn T, Solbach C, Seiter T, Strebhardt K, Holtrich U, Kaufmann M, Identification of high risk breast-cancer patients by gene expression profiling, Lancet. 2002 Jan 12;359(9301):131-2
Ahr A, Holtrich U, Solbach C, Scharl A, Strebhardt K, Karn T, Kaufmann M, Molecular classification of breast cancer patients by gene expression profiling, J Pathol. 2001 Oct;195(3):312-20
Solbach C, Roller M, Fellbaum C, Nicoletti M, Kaufmann M, PTTG mRNA expression in primary breast cancer: a prognostic marker for lymph node invasion and tumor recurrence, Breast. 2004 Feb;13(1):80-1
Kaufmann M, Jonat W, Blamey R, Cuzick J, Namer M, Fogelman I, de Haes JC, Schumacher M, Sauerbrei W; Zoladex Early Breast Cancer Research Association (ZEBRA) Trialists' Group, Survival analyses from the ZEBRA study. goserelin (Zoladex) versus CMF in premenopausal women with node-positive breast cancer, Eur J Cancer. 2003, Aug;39(12):1711-7
Kaufmann M, von Minckwitz G, Smith R, Valero V, Gianni L, Eiermann W, Howell A, Costa SD, Beuzeboc P, Untch M, Blohmer JU, Sinn HP, Sittek R, Souchon R, Tulusan AH, Volm T, Senn HJ, International expert panel on the use of primary (preoperative) systemic treatment of operable breast cancer: review and recommendations, J Clin Oncol. 2003 Jul 1;21(13):2600-8
Solbach C, Roller M, Budischewski K, Loibl S, Nicoletti M, Stegmueller M, Kaufmann M, EGF-R and Her2/neu overexpressing tumors: independent collectives for treatment of breast cancer by specific monoclonal antibody-therapy, J Cancer Res Clin Oncol. 2003 Apr;129(4):250-1. Epub 2003 Apr 23
Loibl S, von Minckwitz G, Weber S, Sinn HP, Schini-Kerth VB, Lobysheva I, Nepveu F, Wolf G, Strebhardt K, Kaufmann M, Expression of endothelial and inducible nitric oxide synthase in benign and malignant lesions of the breast and measurement of nitric oxide using electron paramagnetic resonance spectroscopy, Cancer. 2002 Sep 15;95(6):1191-8
Solbach C, Roller M, Ahr A, Loibl S, Nicoletti M, Stegmueller M, Kreysch HG, Knecht R, Kaufmann M, Anti-epidermal growth factor receptor-antibody therapy for treatment of breast cancer, Int J Cancer. 2002 Oct 1;101(4):390-4
Solbach C, Sterner-Kock A, Roller M, Schnurch HG, Stegmuller M, Caspar-Bell G, Schumm-Draeger PM, Kaufmann M, Knecht R, Antitumor effect of MAb EMD 55900 depends on EGF-R expression and histopathology, Neoplasia. 2002 May-Jun;4(3):237-42
von Minckwitz G, Costa SD, Raab G, Blohmer JU, Eidtmann H, Hilfrich J, Merkle E, Jackisch C, Gademann G, Tulusan AH, Eiermann W, Graf E, Kaufmann M; German Preoperative Adriamycin-Docetaxel and German Adjuvant Breast Cancer Study Groups, Dose-dense doxorubicin, docetaxel, and granulocyte colony-stimulating factor support with or without tamoxifen as preoperative therapy in patients with operable carcinoma of the breast: a randomized, controlled, open phase IIb study, J Clin Oncol. 2001 Aug 1;19(15):3506-15
Holtrich U, Wolf G, Yuan J, Bereiter-Hahn J, Karn T, Weiler M, Kauselmann G, Rehli M, Andreesen R, Kaufmann M, Kuhl D, Strebhardt K, Adhesion induced expression of the serine/threonine kinase Fnk in human macrophages, Oncogene. 2000 Oct 5;19(42):4832-9
Kaufmann M, Bajetta E, Dirix LY, Fein LE, Jones SE, Zilembo N, Dugardyn JL, Nasurdi C, Mennel RG, Cervek J, Fowst C, Polli A, di Salle E, Arkhipov A, Piscitelli G, Miller LL, Massimini G, Exemestane is superior to megestrol acetate after tamoxifen failure in postmenopausal women with advanced breast cancer: results of a phase III randomized double-blind trial. The Exemestane Study Group, J Clin Oncol. 2000 Apr;18(7):1399-411
von Minckwitz G, Costa SD, Eiermann W, Blohmer JU, Tulusan AH, Jackisch C, Kaufmann M, Maximized reduction of primary breast tumor size using preoperative chemotherapy with doxorubicin and docetaxel, J Clin Oncol. 1999 Jul;17(7):1999-2005