Institut für pharmazeutische
Biologie -
Prof. Dr. Theo Dingermann /
Prof. Dr. Rolf Marschalek |
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Expertise
- Genomische Instabilität
durch transposable und retrotransposable
Elemente
- Retrotransposons und andere
mobile genetische Elemente
- Neue Strategien zur Verbesserung
der Integrationsspezifität
von Vektoren für die
Gentherapie
- Dictyostelium als Expressionssystem
für rekombinante Proteine
und Testsystem für Embryotoxizität
- Target-Identifizierung für
bekannte und neue Wirkstoffe
- Akute Leukämien beim
Menschen (Hämato-Onkologie)
- Pathomolekulare Mechanismen
in der Leukämie-Entstehung
- Genetische Instabilität
- Mechanismen der Genegulation
- Expression profiling
- Diagnostik menschlicher
Erkrankungen
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Methoden
und Geräte
- Herstellung monoklonaler
Antikörper
- 2D-Gelelektrophorese
- Massenspektrometrie
- Funktionelle Testsysteme
- Entwicklungstoxikologie
- Targetsuche
- Identifizierung neuer Targetproteine
in unterschiedlichen Geweben
für bekannte und
neue Wirkstoffe (Wirkung,
Metabolisierung, Elimination)
- DNA-Sequenzierung, Klonierung,
Transfektion/Transformation
- Zellkultur von Eukaryonten
(Hefe, Dictyostelium, Maus,
Mensch)
- Protein-Interaktion (Mensch,
Hefe)
- Protein Biochemie / Struktur
von Proteinen / Wirkmechanismen
(Mensch)
- Herstellung rekombinanter
Proteine in Bakterien, Hefe,
Dictyostelium, Mensch
- Molekulare Diagnostik über
PCR / FACS
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Publikationen
- Szafranski K, Dingermann
T, Glöckner G , and Winckler
T. Template jumping by a Dictyostelium
LINE reverse transcriptase
created a SINE-like 5S rRNA
retropseudogene, Mol. Genet.
Genom. 2004 (accepted)
- Classen B, Csávás
M, Borbás A, Dingermann
T, and Zündorf I. Monoclonal
antibodies against an arabinogalactan-protein
from pressed juice of Echinacea
purpurea. Planta Med. 2004
(accepted)
- Bursen A, Moritz S, Gaussmann
A, Dingermann T, Marschalek
R. (2003). Deciphering the
t(4;11) pathomechanism”.
Submitted.
- Keller JH, Karas M, Müller
WE, Volmer DA, Eckert GP,
Tawab MA, Blume HH, Dingermann
T, and Schubert-Zsilavecz
T. Determination of Hyperforin
in Mouse Brain by High-Performance
Liquid Chromatography/Tandem
Mass Spectrometry. Anal. Chem.
2003; 75 (22):6084-608
- Meyer C, Marschalek R. (2003).
Akute Hochrisiko-Leukämie.
BioForum 9/2003, 526-528.
- Schraets D, Lehmann T, Dingermann
Th, Marschalek R. (2003).
MLL mediated transcriptional
gene regulation investigated
by gene expression profiling.
Oncogene 22, 3655-3668
- Lühe A, Hildebrand
H, Bach U, Dingermann T, Ahr
HJ. A New Approach to Studying
Ochratoxin A (OTA)-Induced
Nephrotoxicity: Expression
Profiling in Vivo and in Vitro
Employing cDNA Microarrays.
Toxicol. Sci. 2003; 73:315-328
- Böhme TM, Klein C,
Kreutzmann K, Linder M, Dingermann
T, Dannhardt G, Mutschler
E, Lambrecht G. Structure-activity
relationships of dimethindene
derivatives as new M2-selective
muscarinic receptor antagonists.
J. Med. Chem. 2003, 46:856-86
- Dannat K, Tillner J, Winckler
T, Weiß M, Eger K, and
Dingermann, T. The effects
of medicinal compounds on
the differentiation of the
eukaryotic microorganism Dictyostelium
discoideum: Can this model
be used as a screening test
for reproductive toxicity
in humans? Pharmazie 2003;
58:204-210
- Glöckner G, Eichinger
L, Szafranski K, Pachebat
J, Dear P, Lehmann R, Baumgart
C, Abril JF, Parra G, Guigó
R, Kumpf K, Tunggal, Cox E,
Quail MA, Platzer M, Rosenthal
A, Noegel AA, and the Dictyostelium
Genome Sequencing Consortium
(u.a. Dingermann T, Winckler
T). Sequence and analysis
of chromosome 2 of Dictyostelium
discoideum, Nature 2002; 418:79–85
- König M, Reichel M.
Marschalek R, Haas OA, Strehl
S. A highly specific and sensitive
FISH assay for the detection
of t(4;11)(q21;q23) in acute
leukemias. British Journal
of Haematology 2002; 116,
758-764.
- Friese A, Hell-Momeni K,
Zündorf I, Winckler T,
Dingermann T, and Dannhardt
G. Synthesis and biological
evaluation of cycloalkylidene
carboxylic acids as novel
effectors of Ras/Raf interaction,
J. Med. Chem. 2002; 45:1535-1542
- Beck P, Dingermann T, and
Winckler T. Transfer RNA gene-targeted
retrotransposition of Dictyostelium
TRE5-A into a chromosomal
UMP synthase gene trap, J.
Mol. Biol. 2002; 318:273-285
(MOLEKULARBIOLOGIE)
- Hentschel U, Zündorf
I, Dingermann T, and Winckler
T. On the problem of establishing
the subcellular localization
of Dictyostelium retrotransposon
TRE5-A proteins by biochemical
analysis of nuclear extracts,
Anal. Biochem. 2001; 296:83-91
- Fasching K, Panzer S, Haas
OA, Borkhardt A, Marschalek
R, Griesinger F, Panzer-Grümayer
ER. (2001). The Presence of
N Regions in the Clonotypic
DJ Rearrangements of the Ig
Heavy Chain Genes Indicate
an Exquisitely Short Latency
in t(4;11) Positive Infant
Acute Lymphoblastic Leukemia.
Blood 2001; 98, 2272-2274
- Glöckner G, Szafranski
K, Winckler T, Dingermann
T, Quail M, Cox E, Eichinger
L, Noegel AA, and Rosenthal
A. The complex repeats of
Dictyostelium discoideum,
Genome Res. 2001; 11:585-594
- Winckler T, Trautwein C,
Tschepke C, Neuhäuser
C, Zündorf I, Vogel G,
and Dingermann T. Gene function
analysis by stop codon suppression:
CMBF is a nuclear protein
that supports growth and development
of Dictyostelium amoebae,
J. Mol. Biol. 2001; 305:703-714
- Hensel JP, Gillert E, Fey
GH, Marschalek R. (2001).
Breakpoints of t(4;11) translocations
in the human MLL and AF4 genes
in ALL patients are preferentially
clustered outside of high-affinity
matrix attachment regions.
Journal of Cellular Biochemistry
2001; 82, 299-309
- Reichel M, Gillert E, Angermüller
S, Hensel JP, Heidel F, Lode
M, Leis T, Biondi A, Haas
OA, Strehl S, Panzer-Grümayer
ER, Griesinger F, Beck JD,
Greil J, Fey GH, Uckun FM,
Marschalek R. Biased distribution
of chromosomal breakpoints
involving the MLL gene in
infants versus children and
adults with t(4;11) ALL. Oncogene
2001; 20, 2900-2907
- Von Berg A, Gargallo P,
De Prijck B, Vranckx H, Marschalek
R, Larippa I, Kluin Ph, Schuuring
E, Hagemeijer A. Cryptic t(4;11)
encoding MLL-AF4 due to insertion
of 5‘ MLL sequences
in chromosome 4. A molecular
cytogenetic study. Leukemia2001;
15, 595-600
- Reichel M, Gillert E, Breitenlohner
I, Repp R, Greil J, Beck JD,
Fey GH, Marschalek R. MRD-Meeting
report: „Rapid isolation
of chromosomal breakpoints
from patient with t(4;11)
acute lymphoblastic leukemia:
implications for basic and
clinical research". Leukemia
2001; 15, 286-288.
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Mitarbeiter:
-
Prof. Dr. Theo Dingermann
- Dr. rer. nat. Ilse Zündorf
- Apotheker Christian Klein
- Apothekerin Ute Steine
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HD. Dr. Thomas Winckler
- Dipl. Biol. Monika Baik
- Dipl. Ing. Oliver Siol
- Apotheker Ingo Jennes
- Apothekerin Thanh Chung
- Julia Bosse (TA)
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Prof. Dr. Rolf Marschalek
- Dipl. Biol. Adelheid Bursen
- Dipl. Biol. Anne Gaußmann
- Sabine Jakob (TA)
- Apotheker Sven Moritz
- Dipl. Ing. Claus Meyer
- Dipl. Biol. Sebastian
Scharf
- Dipl. Biol. Björn
Schneider
- Dipl. Chem. Jürgen
Zech
- Jens Rabenstein (TA)
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ZAFES-Ansprechpartner:
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