Institut für pharmazeutische Chemie -
Prof. Dr. Michael Karas

Expertise

Proteomics: Identifizierung und Charakterisierung von Proteinen aus Zellen oder Zellkompatimenten
Massenspektrometrie (ESI und MALDI) von intakten Proteinen, Peptiden, Oligosacchariden, Arzneimitteln und Metaboliten
Verfahrensentwicklung zur Untersuchung von Membranproteinen und Membranprotein- Komplexen
In-vitro Assay für Transkription/Translation für die Suche nach neuen Inhibitoren der bakteriellen Proteinsynthese (Antibiotika)
Verfahrensentwicklung in der Wirkstoffanalytik
Flüssig-Chromatographie (HPLC)
Gel Elektrophorese

Methoden und Geräte

1D/2D-Gel-Electrophorese
Blau-native Gel-Elektrophorese
MALDI TOF Massenspektrometrie
MALDI TOF-TOF Massenspektrometrie
Nano-ESI-MS
HPLC-ESI-MS/MS
µ-bore, Kapillar- und nano-HPLC
TAP Tandem-Affinitätsaufreinigung zur Untersuchung funktioneller Multi-Protein Komplexe

Publikationen

F. Hillenkamp, M. Karas, R.C. Beavis, B.T. Chait, Matrix- Assisted Laser Desorption/Ionization Mass Spectrometry of Biopolymers. Anal. Chem. 63 (1991) 1193A -1203A.
U. Bahr, M. Karas, Differentiation of "Isobaric" Peptides and Human Milk Oligosaccharides by Exact Mass Measurements Using ESI-orthogonal TOF Analysis. Rapid Commun. Mass Spectrom. 13 (1999) 1052-1058.
B. Finke, B. Stahl, A. Pfenninger, M. Karas, H. Daniel, G. Sawatzki, Analysis of High-Molecular-Weight Oligosaccharides from Human Milk by Liquid Chromatography and MALDI-MS. Anal. Chem. 71 (1999) 3755-3762.
M.Abdel Tawab, U. Bahr, B. Danieli, S. Gebhardt, M. Karas, S. Riva, M. Schubert-Zsilavecz, Electrospray Mass Spectrometry with Consecutive Fragmentation Steps (ES-MSn) as a Tool for Rapid and Sensitive Analysis of Ginsenosides and their Galactosyl Derivatives. Helv. Chim. Acta 83 (2000) 739-747.
A. Schmidt, U. Bahr, M. Karas, The Influence of Pressure in the First Pumping Stage on Analyte Desolvation and Fragmentation in Nano-ESI MS. Anal. Chem. 73 (2001) 6040-6046.
A. Schmidt, M. Karas, The influence of Electrostatic Interactions on the Detection of Heme-Globin Complexes in ESI-MS J. Am. Soc. Mass Spectrom. 12 (2001) 1092-1098.
A. Pfenninger, M. Karas, B. Finke, B. Stahl, Structural Analysis of Underivatized Neutral Human-Milk Oligosaccharides in the Negative Ion Mode by Nano-Electrospray MSn (Part 1 : Methodology). J. Am. Soc. Mass Spectrom. 13 (2002) 1331-1340.
A. Pfenninger, M. Karas, B. Finke, B. Stahl, Structural Analysis of Underivatized Neutral Human-Milk Oligosaccharides in the Negative Ion Mode by Nano-Electrospray MSn (Part 2 : Application to Isomeric Mixtures.) J. Am. Soc. Mass Spectrom. 13 (2002) 1341-48.
A. Schmidt, T. Dülks, M. Karas, Effect of different solution flow rate on analyte ion signals in nano-ESI MS, or : When does ESI turn to Nano-ESI? J. Am. Soc. Mass Spectrom. 14 (2003) 492-500.
R. Krüger, M. Karas, Ion Formation in MALDI: The Cluster Ionization Mechanism. Chem. Rev. 103 (2003) 427-439.
C. Corvey, T. Stein, S. Düsterhus, M. Karas, K.-D. Entian, Activation of subtilin precursors by Bacillus subtilis extracellular serine proteases subtilisin (AprE), WprA, and Vpr. Biochem. Biophys. Res. Commun. 304 (2003) 48-54.
K. Ekroos, C. S. Ejsing, U. Bahr, M. Karas, K. Simons, A. Shevchenko. Charting molecular composition of phosphatidylcholines by fatty acid scanning and ion trap MS3 fragmentation. J. Lipid Res. 44 (2003) 2181-2192.
J.-H. Keller, M. Karas, W.E. Müller, D.A. Volmer, G.P. Eckert, M. Abdel-Tawab, H.H. Blume, T. Dingermann, M. Schubert-Zsilavecz, Determination of Hyperforin in Mouse Brain by Rapid High Performance Liquid chromatography/Tandem Mass Spectrometry. Anal. Chem. 75 (2003) 6084-6088.
I. Rais, M. Karas, H.Schägger
Two-dimensional gel elctrophoresis for the isolation of integral membrane proteins and mass spectrometric identification
Proteomics 4 (2004)2567-2571
A.Dörrschuck, A. Schmidt, E. Schnürer, M. Glückmann, C. Albrecht, C. Wölfel, V. Lennerz,
A. Lifke, C. Di Natale, E. Ranieri, C. Huber, M. Karas, W. Herr
CD8+ cytotoxic T lymphocytes isolated from allogeneic healthy donors recognize HLA-class Ia/Ib-associated renal carcinoma antigens with ubiquitous or restricted tissue expression Blood 104 (2004) 2591-99
M. Aivaliotis, C. Corvey, I. Tsirogianni, M. Karas, G. Tsiotis
Membrane proteome analysis of the green-sulfur bacterium Chloropium tepidum
Electrophoresis 25 (2004) 3468-3474.
S. Hofmann, M. Glückmann, S. Kausche, A. Schmidt, C. Corvey, R. Lichtenfels, C. Huber, C.Albrecht, M. Karas, W. Herr. Rapid and sensitive identification of major histocompatibility complex class I-associated tumor peptides by nanoLC MALDI MS/MS mass spectrometry. Mol Cell Proteomics 4 (2005) 1888-97.
Fandino, I. Rais, M. Vollmer, H. Elgass, H. Schägger, M. Karas
LC-nanospray-MS/MS analysis of hydrophobic proteins from membrane protein complexes isolated by blue-native electrophoresis J. Mass Spectrom. 40 (2005) 1223-1231
C. Corvey, T. Stein, P. Kötter, K.D. Entian, M. Karas
Carbon source dependent assembly of the Snf1p kinase complex in Candida albicans. J. Biol. Chem. 280 (2005) 25323-30.